@article{bibcite_5111,
  keywords = {Nanobiotechnology, Regeneration, Stem-cell biotechnology},
  author = {Yeon-Woo Cho and Min-Ji Kang and Joon-Ha Park and Yun-Sik Eom and Tae-Hyung Kim},
  title = {Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal{\textendash}organic framework-coated nanoline arrays},
  abstract = {Improved in vitro models are needed for regenerative therapy and drug screening. Here, we report on functionally aligned nanoparticle-trapped nanopattern arrays for spatially controlled, precise mesenchymal stem cell differentiation on a single substrate. The arrays comprise nanohole and nanoline arrays fabricated through interference lithography and selectively capture of UiO-67 metal{\textendash}organic frameworks on nanoline arrays with a 99.8\% efficiency using an optimised asymmetric spin-coating method. The UiO-67 metal{\textendash}organic frameworks contain three osteogenic differentiation factors for sustained release over four weeks. The combination of differentiation factors and patterned array allows for generation of adipocytes, osteoblasts, and adipocyte{\textendash}osteoblast mixtures on nanohole arrays, nanoline arrays, and at the nanohole{\textendash}nanoline interface, respectively, with mature osteoblasts exhibiting higher marker expression and mineralisation. The sustained release patterned array holds potential for constructing advanced therapeutic and disease state in vitro cellular models.},
  year = {2025},
  journal = {Nature Communications},
  volume = {16},
  pages = {1389},
  month = {2025-02-06},
  issn = {2041-1723},
  url = {https://www.nature.com/articles/s41467-025-56373-0},
  doi = {10.1038/s41467-025-56373-0},
  language = {en},
}