@article{bibcite_4806,
  keywords = {Biomedical Engineering, Gastrointestinal models, intestinal stem cells, Lab-on-a-chip, Stem-cell biotechnology},
  author = {Moritz Hofer and Maria A. Duque-Correa and Matthias P. Lutolf},
  title = {Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection},
  abstract = {Organoids for modelling the physiology and pathology of gastrointestinal tissues are constrained by a poorly accessible lumen. Here we report the development and applicability of bilaterally accessible organoid-derived patterned epithelial monolayers that allow the independent manipulation of their apical and basal sides. We constructed gastric, small-intestinal, caecal and colonic epithelial models that faithfully reproduced their respective tissue geometries and that exhibited stem cell regionalization and transcriptional resemblance to in vivo epithelia. The models{\textquoteright} enhanced observability allowed single-cell tracking and studies of the motility of cells in immersion culture and at the air{\textendash}liquid interface. Models mimicking infection of the caecal epithelium by the parasite Trichuris muris allowed us to live image syncytial tunnel formation. The enhanced observability of bilaterally accessible organoid-derived gastrointestinal tissue will facilitate the study of the dynamics of epithelial cells and their interactions with pathogens.},
  year = {2024},
  journal = {Nature Biomedical Engineering},
  pages = {1-11},
  month = {2024-12-04},
  issn = {2157-846X},
  url = {https://www.nature.com/articles/s41551-024-01313-4},
  doi = {10.1038/s41551-024-01313-4},
  language = {en},
}