@article{bibcite_1771,
  keywords = {3D bioprinting, Angiotensin-Converting Enzyme 2, Animals, Antiviral Agents, Bioprinting, COVID-19, Humans, organoids, SARS-CoV-2, Spheroids, Cellular, Tissue engineering, Tissue Scaffolds, Tissue engineering},
  author = {Bruna A. G. de Melo and Julia C. Benincasa and Elisa M. Cruz and Juliana Terzi Maricato and Marimelia A. Porcionatto},
  title = {3D culture models to study SARS-CoV-2 infectivity and antiviral candidates: From spheroids to bioprinting},
  abstract = {The pandemic caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is receiving worldwide attention, due to the severity of the disease (COVID-19) that resulted in more than a million global deaths so far. The urgent need for vaccines and antiviral drugs is mobilizing the scientific community to develop strategies for studying the mechanisms of SARS-CoV-2 infection, replication kinetics, pathogenesis, host-virus interaction, and infection inhibition. In this work, we review the strategies of tissue engineering in the fabrication of three-dimensional (3D) models used in virology studies, which presented many advantages over conventional cell cultures, such as complex cytoarchitecture and a more physiological microenvironment. Scaffold-free (spheroids and organoids) and scaffold-based (3D scaffolding and 3D bioprinting) approach allow the biofabrication of more realistic models relevant to the pandemic, to be used as in~vitro platforms for the development of new vaccines and therapies against COVID-19.},
  year = {2021},
  journal = {Biomedical Journal},
  volume = {44},
  pages = {31-42},
  month = {2021-03},
  issn = {2320-2890},
  doi = {10.1016/j.bj.2020.11.009},
  language = {eng},
}