@article{bibcite_1531,
  keywords = {Animal Testing Alternatives, Animals, Coculture Techniques, Gastrointestinal Microbiome, Host Microbial Interactions, Humans, Intestinal Mucosa, Mice, Microbial Interactions, Microchip Analytical Procedures, microfluidics, Models, Animal, organoids, Precision Medicine, bacteria, Co-culture, Epithelium, host, infection, intestine, in~vitro models, microbe, microbiota, organ-on-a-chip, organoid, virus},
  author = {Jens Puschhof and Cayetano Pleguezuelos-Manzano and Hans Clevers},
  title = {Organoids and organs-on-chips: Insights into human gut-microbe interactions},
  abstract = {The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in~vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in~vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures.},
  year = {2021},
  journal = {Cell Host \& Microbe},
  volume = {29},
  pages = {867-878},
  month = {2021-06-09},
  issn = {1934-6069},
  doi = {10.1016/j.chom.2021.04.002},
  language = {eng},
}